Fluorescence turn-on reactions of bicyclononynes

We show in our new work published in the special “Early Career Series” issue of ChemPlusChem that bicyclononynes (BCN) in reaction with tetrazines containing electron-donating substituents form fluorescent dyes. This fluorogenic reaction enables bioimaging with excellent signal to noise ratio.

• S. J. Siegl, J. Galeta, R. Dzijak, M. Dračínský, M. Vrabel, Bioorthogonal Fluorescence Turn‐On Labeling Based on Bicyclononyne−Tetrazine Cycloaddition Reactions that Form Pyridazine Products, ChemPlusChem 2019, 84, 493, https://doi.org/10.1002/cplu.201900176