Genetic incorporation of clickable amino acids in B. subtilis
13 February 2020
In collaboration with the group of Sabine Schneider at LMU Munich, we present in this paper an optimized expression system for production of norbornene-containing single chain antibody. The antibody containing the unnatural amino acid is fully functional and can be used for the attachment of other useful functional groups by click chemistry.
- C. M. Scheidler, M. Vrabel, and S. Schneider, Genetic Code Expansion, Protein Expression, and Protein Functionalization in Bacillus subtilis, ACS Synthetic Biology 2020 9 (3), 486-493, https://doi.org/10.1021/acssynbio.9b00458